منابع مشابه
The specificity of cathepsin D.
Bovine spleen cathepsin D has been partially purified for studies of its specificity. Isoelectric focusing of the purest preparation obtained indicated the presence of three molecular forms having approximately the same specific activity, but differing in their isoelectric points. Gel chromatography of this preparation on Sephadex G-100 gave an apparent molecular weight of about 42,000, and its...
متن کاملAction of Cathepsin C on Dipeptide Esters *
In earlier studies"2 on the purification and properties of beef spleen cathepsin C, it was found that at pH 5 this proteolytic enzyme catalyzes the hydrolysis, not only of the CO-NH bonds of sensitive substrates, but also of the ester linkage of a-L-glutamyl-L-tyrosine ethyl ester. In view of the subsequent finding4 that cathepsin C exhibits pronounced specificity toward dipeptide derivatives (...
متن کاملCathepsin B carboxydipeptidase specificity analysis using internally quenched fluorescent peptides.
We have examined in detail the specificity of the subsites S1, S2, S1' and S2' for the carboxydipeptidase activity of cathepsin B by synthesizing and assaying four series of internally quenched fluorescent peptides based on the sequence Dnp-GFRFW-OH, where Dnp (2,4-dinitrophenyl) is the quenching group of the fluorescence of the tryptophan residue. Each position, except the glycine, was substit...
متن کاملTransamidation reactions catalyzed by cathepsin C.
Previous publications from this laboratory (l-3) have described the catalysis of transamidation reactions by the proteinases papain, ficin, and crystalline chymotrypsin. In addition, preliminary experiments were reported on replacement reactions catalyzed by beef spleen cathepsin C, an intracellular endopeptidase of animal tissues, which resembles pancreatic chymotrypsin in its specificity. The...
متن کاملRole of cathepsin A and cathepsin C in the regulation of glycosidase activity.
Increased tissue activity of cathepsin A and cathepsin C can be observed in many pathological conditions. It is associated with an enhanced degradation of glycosaminoglycans, proteoglycans, and glycoproteins, and results in their decreased tissue content. Cathepsin C releases the glycosidases from complexes formed with cathepsin A, and reinstates their activity. In this review a current state o...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1956
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)65871-5